Each fiber in a multiple-fiber catheter sequentially delivered excitation light and collected fluorescent emission to yield a fluorescence spectrum for that pixel. One of the early examples in medical optics of hyperspectral imaging was the multifiber fluorescence probe developed by Michael Feld's group at M.I.T. Such an image is sometimes called a data cube or hypercube, in which the x-y plane shows the tissue structure and the z-axis encodes the wavelength-dependent information. Hyperspectral imaging is the technique of acquiring two-dimensional images in which each pixel has spectral information.
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